Bioworld Technology

Bioworld Technology is manufacturer of over 15,000 highly purified Monoclonal and Polyclonal Antibodies, Peptides, Proteins, and other related Research Products. Their products are used in all biological fields. An important focus in research taking place today is in work investigating Signal transduction pathways, Cardiac markers, Neuroscience as well as Stem cell research. Bioworld Technology is committed to providing customers with innovative research tools and to helping scientists determine the mechanisms of cell function and disease.
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RecombinantNT-4,Human BK0144

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Specifications

size	10μg / 50μg

Source:

Escherichia coli.

Molecular Weight:

28.1 kDa, a noncovalently linked homodimer,of two 14.0 kDa polypeptide monomers.

Purity:

> 95% by SDS-PAGE and HPLC analyses.

Biological activity:

ED50 < 5.0 μg/ml, measured by a cell proliferation assay using C6 cells, corresponding to a specific activity of > 2.0× 10ˆ2 units/mg.

AA Sequence:

MGVSETAPA SRRGELAVCD AVSGWVTDRR TAVDLRGREV EVLGEVPAAG GSPLRQYFFE TRCKADNAEE GGPGAGGGGC RGVDRRHWVS ECKAKQSYVR ALTADAQGRV GWRWIRIDTA CVCTLLSRTG RA

Endotoxin:

< 0.3 EU/μg, determined by LAL method.

Formulation:

Lyophilized after extensive dialysis against 50mM acetic acid.

Background:

Neurotrophin-4 (NT-4), also known as NT-5, is a neurotrophic factor structurally related to β-NGF, BDNF, and NT-3. Human NT-4 shares 48 - 52% aa sequence identity with human β-NGF, BDNF, and NT-3.Neurotrophins have six conserved cysteine residues that are involved in the formation of three disulfide bonds. NT-4 is expressed highest levels in prostate, lower levels in thymus, placenta, and skeletal muscle. NT-4 binds and induces receptor dimerization and activation of TrkB. NT-4 can signal through TrkB receptors and promotes the survival of peripheral sensory sympathetic neurons.Recombinant human Neurotrophin-4 (rhNT-4) produced in E.coli is a noncovalently linked homodimer containing two non-glycosylated polypeptide chains of 131 amino acids. A fully biologically active molecule, rhNT-4 has a molecular mass of 28.1kDa analyzed by reducing SDS-PAGE and is obtained by proprietary chromatographic techniques at GenScript.

Usage:

This material is offered by USA Bioworld biotech for research, laboratory or further evaluation purposes. For research use only.

Physical Appearance:

Sterile Filtered White lyophilized (freeze-dried) powder.

Reconstitution:

Reconstituted in 50mM acetic acid or ddH2O at 50 μg/ml.

Storage:

Lyophilized recombinant human Neurotrophin-4 (rhNT-4) remains stable up to 6 months at -80°C from date of receipt. Upon reconstitution, rhNT-4 should be stable up to 2 weeks at 4°C or up to 3 months at -20°C.