Bioworld Technology

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RecombinantNAP-2/CXCL7,Rat BK0143

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Specifications

size	5μg / 25μg / 1mg

Source:

Escherichia coli.

Molecular Weight:

6.9 kDa, observed by reducing SDS-PAGE.

Purity:

> 95% as analyzed by SDS-PAGE.

Biological activity:

The EC50 value of Rat NAP-2/CXCL7on Caˆ2+ mobilization assay in CHO-K1/Gα15/rCXCR2 cells (human Gα15 and Rat CXCR2 stably expressed in CHO-K1 cells) is less than 200 ng/ml.

AA Sequence:

IELRCRCTNT LSGIPLNSIS RVNVFRPGAH CDNVEVIATL KNGKEVCLDP TAPMIKKIVK KI

Endotoxin:

< 0.2 EU/μg, determined by LAL method.

Formulation:

Lyophilized after extensive dialysis against PBS.

Background:

Rat NAP-2/CXCL7 is a small cytokine belonging to the CXC chemokine family. It is an isoform of Beta-Thromboglobulin or Pro-Platelet basic protein (PPBP). Similar to other ELR domain containing CXC chemokines such as IL-8 and the GRO proteins, NAP-2 has been shown to bind CXCR-2 and recruit and active ateneutrophils through chemotaxis. Although CTAP-III, β-TG and PBP represent amino-terminal extended variants of NAP-2 and possess the same CXC chemokine domains, these proteins do not exhibit NAP-2 activity. NAP-2 stimulates various processes including mitogenesis, synthesis of the extracellular matrix, glucose metabolism and synthesis of plasminogen activator. Recently, it has been shown that the additional amino-terminal residues of CTAP-III mask the critical ELR receptor binding domain that is exposed on NAP-2 and may account for lack of NAP-2 activity.Recombinant Rat NAP-2 /CXCL7 produced in E.coli is a single non-glycosylated polypeptide chain containing 62 amino acids. A fully biologically active molecule, rrNAP-2/CXCL7 has a molecular mass of 6.9 kD analyzed by reducing SDS-PAGE and is obtained by chromatographic techniques at GenScript.

Usage:

This material is offered by USA Bioworld biotech for research, laboratory or further evaluation purposes. For research use only.

Physical Appearance:

Sterile Filtered White lyophilized (freeze-dried) powder.

Reconstitution:

Reconstituted in ddH2O or PBS at 100 μg/ml.

Storage:

Lyophilized recombinant Rat NAP-2/CXCL7 remains stable up to 6 months at -80°C from date of receipt. Upon reconstitution, Rat NAP-2/CXCL7 should be stable up to 1 week at 4°C or up to 3 months at -20°C.