Alternative names:

Angiotensin-converting enzyme 2; Angiotensin-converting enzyme homolog; ACEH; Angiotensin-converting enzyme-related carboxypeptidase; ACE-related carboxypeptidase; Metalloprotease MPROT15; Processed angiotensin-converting enzyme 2; ACE2

Host:

Human

Immunogen:

Reactivity:

293F

Description:

PBS,pH7.4

Specificity:

AA Gln 18 - Ser 740 (Accession # Q9BYF1-1)

Bio-MW:

Applications:

ELISA WB

Application dilution:

The protein has a calculated MW of 111.7 kDa. The protein migrates as 125-150 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation. The L391G(Leucine to glycine at amino acid position 391) mutant human Ace2 protein has been produced in a mammalian expression systems.

Purification & Purity:

The protein was purified from 293F and the purity is > 95% (by SDS-PAGE).

Storage & Stability:

Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

Background:

Angiotensin-converting enzyme 2 (ACE2) is also known as ACEH (ACE homolog), is an integral membrane protein with considerable homologous to ACE, which belongs to the peptidase M2 family. ACE2 is an exopeptidase that catalyses the conversion of angiotensin I to the nonapeptide angiotensin, or the conversion of angiotensin II to angiotensin 1-7. ACE2 may be an important regulator of heart function. In case of human coronaviruses SARS and HCoV-NL63 infections, ACE-2 serve as functional receptor for the spike glycoprotein of both coronaviruses. ACE2 is activated by chloride and fluoride, but not bromide and Inhibited by MLN-4760, cFP_Leu, and EDTA, but not by the ACE inhibitors linosipril, captopril and enalaprilat. ACE2 is active from pH 6 to 9, and the optimum pH is 6.5 in the presence of 1 M NaCl.

Note:

For research use only, not for use in diagnostic procedure.